|
NemaBLAST - Map your sequence to our various transcript database collections
Versus reads grouped by library
This selection is typically used to search for your sequence of
interest amongst a custom selection of libraries from species of interest. For example,
you may search your query against Trichinella spiralis cDNAs, but exclude all libraries
except the adult libraries "Trichinella spiralis adult pAMP1 v1" and "Trichinella spiralis adult SL1 TOPO v1".
Select this option to align your nucleotide or protein sequence
against databases comprising EST reads organized into their library of origin. After
selection the organisms you would like to include, you will be given the chance to
exclude specific libraries from the database. For information on specific libraries
please use our Library Details page.
|
Versus transcript contigs, isotigs & genes
This selection is typically used to screen for the presence or
absence of your query across a spectrum of organisms.
Select this option to align your nucleotide or protein sequence
against databases comprising EST assembly contigs, gene CDS and/or transcript assembly isotigs organized by
species. Databases in this set contain assembly contigs/isotigs constructed from different types
of input data as well as CDS from final genesets. Each database can consist of contigs/isotigs built from
input cDNA reads of several types or from CDS associated with finalized genesets. Names of the form
"Acan_isotig11379" are isotigs constructed by a newbler assembly of Roche/454 cDNA data. Names in
the form of "TS05971" are older contigs built from Sanger-sequencing based EST reads, typically
assembled with Phrap. Names in the form of "CDS_Bmal|BM05276" are CDS of final genesets. Other naming
formats that may appear indicate a contig and/or isotig that was imported from an external
source, and may vary in their composition. For both Roche/454 and Sanger-sequence based
assemblies as well as CDS genesets, the prefixes (first 4 letters for 454 data, and first 2 letters for Sanger
data) indicate the species. For a list of these prefixes, please look in our
NemaGene FAQ
in the "Index of prefixes" section.
|
A Note on NemaBLAST
The NemaBLAST pages use WU-BLAST 2.0 (Gish, W. 1994-2002). Washington
University BLAST (WU-BLAST) version 2.0 is a powerful software
package for gene and protein identification, using sensitive and
selective similarity searches of protein and nucleotide
sequence databases. The feature list for WU-BLAST 2.0 is large,
please visit http://blast.wustl.edu/ for more information
on this software package
|
NemaBlast OFFLINE indefinitely - Please select what you'd like to BLAST against: |
|
|
|
|