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Identification of 22 unique proteins from Oesophagostomum dentatum

O. dentatum L3 were treated with hydrolase inhibitors to prevent the L3-to-L4 molt. Total larval worm proteins were separated by 2-D gel electrophoresis, and the protein profiles of treated and untreated worm populations were compared. Significantly differentially expressed spots were analyzed by MALDI-TOF-MS/MS. Twenty-two unique proteins were identified and annotated.

Selected Tables & Figures from paper:

Figure 2: Two-dimensional electrophoretic gel of O. dentatum L3s displaying protein spots, non wild-type RNAi phenotypes in Caenorhabditis elegans linked to gene homologues in O. dentatum and the effect of inhibitors (i.e. P, o-phenanthroline; S, sodium fluoride; I, iodoacetamide; E, 1,2 epoxy-3-(pnitrophenoxy)-propane) on protein expression in development-inhibited larvae (v,down-regulation; ^, up-regulation; <->, no difference; -, not studied).


Table 2: Annotation of the different protein spots inferred for O. dentatum and MALDI-TOF-MS/MS results.

Publication:

Proteomic analysis of Oesophagostomum dentatum (Nematoda) during larval transition, and the effects of hydrolase inhibitors on development.
Ondrovics, M. et al. PLoS One 8, e63955, doi:10.1371/journal.pone.0063955 (2013).



 
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