Home : Sratti proteomics 1

Amazon awards research credit to support!

[July.10.2018] is now hosted on Amazon!

Bioinformatics Workshop for Helminth Genomics (2015) class materials now freely available!

NTDs get some well deserved coverage outside the usual channels: Financial Times Special Report: Neglected Tropical Diseases

Identification of stage-specific proteases in S.ratti

Excretory-secretory proteins from S. ratti infective L3, free-living stages, and parasitic adult females were collected and separated by 1-D gel electrophoresis; gel slices were analyzed by LC-MS. A total of 586 proteins were identified (450 from iL3, 217 from free-living stages, and 335 from parasitic females) and annotated.

Selected Figures from paper:

Figure 1: One-dimensional SDS-PAGE of E/S proteins from infective larvae, parasitic females and free-living stages showing after Coomassie stain characteristic protein band patterns of the respective stages.

Figure 2: Venn diagram showing the distribution of the identified S. ratti E/S proteins of the studied developmental stages: iL3, pF and flS.


Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti--identification of stage-specific proteases
Soblik H, Younis AE, Mitreva M, Renard BY, Kirchner M, Geisinger F, Steen H, Brattig NW. Mol Cell Proteomics. 2011 Dec;10(12):M111.010157. doi: 10.1074/mcp.M111.010157.

Supplemental Data:

ES products from iL3
ES products from free living stages
ES products from parasitic females
ES products from all stages v4.0(AWS)    Copyright Statement
  User support forum User Support
The Genome Institute Washington University School of Medicine